Nucleoporin gene expression in Xenopus tropicalis embryonic development
Developmental Expression Pattern | Published: 6 July 2016
Nooreen Reza, Mustafa K. Khokha and Florencia Del Viso*
Program in Vertebrate Developmental Biology. Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, CT, USA
Nucleoporins (nups) compose the structure of the nuclear pore complex (NPC) of all cells, but several studies have illuminated nucleoporins’ additional roles in development and the cell cycle. However, a comprehensive study of nupexpression in embryonic development has not yet been reported. We synthesized antisense probes for all nupgenes and used whole-mount in situ hybridization techniques to determine the expression pattern of all members of the nupfamily of genes at three different developmental stages in Xenopus tropicalis. We found that the expression of nupswas not ubiquitous in embryos, but was localized to specific and distinguishable anatomical structures at all three stages tested. We also found that the expression patterns for nupswithin the same subcomplexes were not necessarily identical. Thus, nupexpression is subject to a significant level of regulation during development. These results provide new information for functional studies of nupsto unravel their roles in embryonic development.
nups, NPC, nuclear pore complex, in situ hybridization