The International Journal of Developmental Biology

Int. J. Dev. Biol. 45: S93 - S94 (2001)

Vol 45, Issue S1

D-4 and D-1/D-5 dopamine receptors interact synergistically in the rat striatum

Published: 1 June 2001

S Trias, A Rivera, M Megias, A Penafiel, F Marin-Giron, A De la Calle

Univ Malaga, Dept Cell Biol, E-29071 Malaga, Spain

Abstract

D1-like (D-1 and D-5) and D-2-like (D-2, D-3 and D-4) dopamine receptor subtypes interact synergistically in many paradigms, such as dopamine (DA) agonist-stimulated motor behaviour and striatal c-Fos expression in intact and DA-depleted striatum. However, it is not clear which subtypes of dopamine receptor are involved in these process. We now report the implication of D-4 receptor in D1/D2 interactions by examining the effects of the D-1/D-5 agonist SKF 38393 and the D-4 agonist PID 168,077 on the expression of c-Fos in rats with unilateral 6-Hydroxydopamine(6-OHDA) lesions of the nigroestriatal pathway. Systemic administration of PD 168,077 led to a higher dose-dependent induction of c-Fos in the lesioned striatum as compared with the contralateral portion. In all cases, c-Fos expression pattern was heterogeneous, being more abundant in the medial part. A low dose of SKF 38393 or PID 168,077 alone produce little induction of c-Fos, but combination of both agonists produced a synergistic effect on c-Fos expression. In this case c-Fos-positive nuclei pattern was heterogeneous in the latero-medial axis and occurred primarily in patches. Furthermore, we have demonstrated that activated cells are medium-sized projecting neurons. This finding suggest that D-4 dopamine receptors play an important role in D1/D2 interactions occurred in an animal model of Parkinson's disease.

Keywords

Stimulation, expression, neurons

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