The International Journal of Developmental Biology

Volume 67 > Issue 4

Cover Vol. 67 N. 4

Cover Legend

Chick embryo at day 8 of development in in ovo windowing culture, an ideal time point to initiate angiogenesis assay. For further details, see the article by Senrung et al. in this issue, pp. 115-135.

Meeting Report


Developmental Biology: from genes to functional organism – news from the 3rd Meeting of the Visegrád Group Society for Developmental Biology

Ewelina Trela, Agnieszka Walewska

Int. J. Dev. Biol. (2023) 67: 109-114


The third meeting of the Visegrád Group Society for Developmental Biology (V4SDB) was held on September 8th-10th, 2023 in Warsaw, Poland. It was a continuation of previous meetings, the first organized in the Czech Republic in 2018 and the second in Hungary in 2021. Similarly to the previous meetings, the organizers created a friendly platform for networking and science sharing. The conference gathered an excellent group of 160 researchers working on various animal models, who during lecture and poster sessions discussed a broad range of subjects, including early embryonic development, organogenesis, genetic and epigenetic control of developmental processes, stem cells and regeneration, cellular dynamics and migration in developmental biology, and in vitro models in development and disease. Additionally, two satellite events were organized: the Young Developmental Biologists’ Forum, which gave young researchers an opportunity to share and promote their work and to participate in hands-on courses, and an outreach initiative “Developmental Biology for Everyone”, which presented different aspects of developmental biology to a broad audience.

Chick chorioallantoic membrane: a valuable 3D in vivo model for screening nanoformulations for tumor antiangiogenic therapeutics

Anna Senrung, Tanya Tripathi, Divya Janjua, Sunita Kumari Yadav, Arun Chhokar, Nikita Aggarwal, Joni Yadav, Apoorva Chaudhary, Udit Joshi, Pallavi Sethi, Alok Chandra Bharti

Int. J. Dev. Biol. (2023) 67: 115-135


Drug discovery is an extensive process. From identifying lead compounds to approval for clinical application, it goes through a sequence of labor-intensive in vitro, in vivo preclinical screening and clinical trials. Among thousands of drugs screened only a few get approval for clinical trials. Furthermore, these approved drugs are often discontinued due to systemic toxicity and comorbidity at clinically administered dosages. To overcome these limitations, nanoformulations have emerged as the most sought-after strategy to safely and effectively deliver drugs within tumors at therapeutic concentrations. Most importantly, the employment of suitably variable preclinical models is considered highly critical for the therapeutic evaluation of candidate drugs or their formulations. A review of literature from the past 10 years on antiangiogenic nanoformulations shows the employment of limited types of preclinical models mainly the 2-dimensional (2D) monolayer cell culture and murine models as the mainstay for drug uptake, toxicity and efficiency studies. To top it all, murine models are highly expensive, time-consuming and require expertise in handling them. The current...


Strontium-doped hydroxyapatite and its role in osteogenesis and angiogenesis

Ling Ran, Lishuang Liu, Jingjing Gao, Yang Pan, Murugan Ramalingam, Xiaoyu Du, Ying Liu, Lijia Cheng, Zheng Shi

Int. J. Dev. Biol. (2023) 67: 137-146


For the past 50 years, hydroxyapatite (HA) has been widely used in bone defect repair because it is the main inorganic component of the mineral phase of a human bone. Extensive preclinical and clinical studies have shown that strontium (Sr) can safely and effectively help prevent and treat bone diseases, including osteoporosis. These findings have resulted in the concept of integrating Sr and HA for bone disease management. The doped Sr can improve the physicochemical properties of HA and enhance its angiogenic and bone regeneration ability. Nevertheless, no study has reviewed the design strategy of Sr-doped HA (Sr-HA) to understand its biological roles. Therefore, in this article, we review recent developments in Sr-HA preparation and its effect on osteogenesis and angiogenesis in vitro and in vivo along with key suggestions for future research and development.
Original Article


The stem cell transcription factor ZFP296 transforms NIH3T3 cells and promotes anchorage-independent growth of cancer cells

Yumi Mizoue, Tomomi Ikeda, Takako Ikegami, Oleksandra Riabets, Yoshie Oishi, Morikuni Tobita, Hidenori Akutsu, Koichi Hattori, Beate Heissig, Hiroshi Koide

Int. J. Dev. Biol. (2023) 67: 147-153


Cancer cells and embryonic stem (ES) cells share several biological properties, suggesting that some genes expressed in ES cells may play an important role in cancer cell growth. In this study, we investigated the possible role of zinc finger protein 296 (ZFP296), a transcription factor expressed in ES cells, in cancer development. First, we found that overexpression of Zfp296 in NIH3T3 mouse fibroblasts induced two phenomena indicative of cell transformation: enhanced proliferation under low-serum conditions and anchorage-independent growth. We also found that Zfp296 expression was upregulated in the tumor area of a mouse model of colon carcinogenesis. In addition, the expression levels of ZFP296 in various human cell lines were generally low in normal cells and relatively high in cancer cells. Finally, using a soft agar assay, we found that overexpression of ZFP296 promoted the anchorage-independent growth of cancer cells, while its knockdown had the opposite effect. Overall, these results suggest a possible role of the ES-specific transcription factor ZFP296 in cancer.