The International Journal of Developmental Biology

Volume 66 > Issue 4-5-6

Cover Vol. 66 N. 4-5-6

Cover Legend

Trunk neural crest cells give rise to different types of mesenchymal cells both in vivo and in vitro. For further details, see the Review article by Rodrigues-Da-Silva et al. in this issue, pp. 317-331.

Short Communication

Correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in mouse

Ya-Ting Sun, Ai-Zhen Zhu

Int. J. Dev. Biol. (2022) 66: 305-309


The aim of this study was to investigate the correlation between CDK1 protein and CDK1 mRNA during oocyte maturation in vivo in mouse. GV, GVBD, MI and MII oocytes were obtained from mice, respectively. Western blot validated that the CDK1 protein expression increased continuously and significantly with oocyte maturation in vivo (P<0.05). Real-time qRT-PCR showed that CDK1 mRNA expression was down-regulated significantly during transformation from GV to MI stages (P<0.05), and up-regulated significantly during transformation from MI to MII stages (P<0.05). The level of CDK1 mRNA peaked at MII stages. Spearman correlation analysis indicated that CDK1 protein expression was poor correlation with CDK1 mRNA expression during oocyte maturation in vivo (R=0.200). This finding suggested that the increase of CDK1 protein during oocyte maturation in vivo was not entirely caused by the change of transcription level. The results provide new food for thought for further research on the molecular mechanism of oocyte maturation in vivo.


Epithelial-endothelial transition and endothelial-mesenchymal transition

Domenico Ribatti

Int. J. Dev. Biol. (2022) 66: 311-316


The movement of continuous sheets of epithelial cells occurs during embryonic development, tissue repair, and cancer. Common to cellular and molecular principles of collective cell migration, invading cancers seem to reactivate embryonic pathways and patterns of cell movement. Epithelial cells possess the capability to become mesenchymal cells in a process called epithelial mesenchymal transition (EMT), which has been extensively studied and described. The aim of this article is to summarize the most recent literature data concerning less known epithelial-endothelial transition and endothelial-mesenchymal transition.


The mesenchymal potential of trunk neural crest cells

Monica Alves Rodrigues-Da-Silva, Guilherme de Espindola da Silveira, Clarissa Reginato Taufer, Giordano Wosgrau Calloni

Int. J. Dev. Biol. (2022) 66: 317-331


It has long been held that the main difference between cranial and trunk neural crest (CNC and TNC, respectively) was the potential of CNC to originate mesenchymal cell types, especially skeletogenic. This is an age-old question that continues to challenge researchers, even today. Unfortunately, to date, no consensus has concluded the extent of TNC mesenchymal potential, nor has a systematic review been conducted to organize current knowledge about this fascinating question. However, the number of studies related to this question have expanded and deepened considerably in the last few years thanks to several new different species of vertebrates employed, the generation of transgenic animal strains, the combination of cell markers, and also the improvement of cell culture conditions through the use of different substrates and signaling molecules. Therefore, this review summarizes the literature showing that TNCCs can generate a broad range of mesenchymal cell types, including skeletogenic. This potential can be unveiled by certain favorable in vitro conditions, but it also seems to be expressed in some animal structures in vivo, to which TNCCs contribute. We also...


Intraflagellar transport 20 cilia-dependent and cilia-independent signaling pathways in cell development and tissue homeostasis

Fu-Chang Jin, Ming-Hui Zhou, Jing-Jing Chen, Yi Lin, Qi-Wei Zhang, Qiu-Xian Xu, Chang-Chun Zhang, Zhen-Gang Zhang

Int. J. Dev. Biol. (2022) 66: 333-347


Intraflagellar transport (IFT) is an essential condition for ciliogenesis. The primary cilia protrude like antennae and act as chemical or mechanical sensory organelles that coordinate specific receptor localization and signal transduction. IFT20 is the smallest molecule in IFT complex B, which is located in both the cilia and the Golgi complex. Recent studies have shown that IFT20 is a key molecule in multiple signaling pathways. Importantly, in the function of IFT20, signal transduction is not restricted to cilia, but is also involved in non-ciliary functions. Here we summarize current knowledge regarding IFT20-mediated signaling pathways and their relationship with cell development and tissue homeostasis, and analyse the cilia-dependent and cilia-independent mechanisms of IFT20 coordinated signaling pathways and potential crosstalk between the mechanisms. This review provides a comprehensive perspective on IFT20 coordinates signaling mechanisms in cell development and tissue homeostasis.

Exploring the sister cells of embryo sac: developmental and functional attributes

Inderdeep Kaur, Monika Koul

Int. J. Dev. Biol. (2022) 66: 349-358


Synergids are metabolically dynamic cells of the egg apparatus and represent an important component of the female gametophyte. Besides directing the growth of the pollen tube towards the micropylar end of the embryo sac, these ephemeral structures make room for the pollen tube cytoplasm. The nature of chemotrophic substances that direct the growth of the pollen tube, the mechanism of degeneration of one of the synergids before fertilization and the molecular aspects of synergid morphogenesis have been studied in detail. Research carried out on model systems such as Arabidopsis, Brassica, Capsella, Triticum and Torenia has expanded our understanding of the molecular regulation of the pollen tube journey, its guidance and navigation in the pistil. Recently, the critical role of the central cell in fertilization and prevention of polytubey has also been thoroughly investigated. Interesting aspects that lead to degeneration of synergids, and the factors governing degeneration, including molecular aspects, have produced a paradigm shift in the understanding of these intriguing units. Sophisticated confocal microscopy, live cell imaging, and molecular tools have helped...