The International Journal of Developmental Biology

Int. J. Dev. Biol. 67: 19 - 25 (2023)

Vol 67, Issue 1

Single-cell transcriptome profiling reveals distinct expression patterns among genes in the mouse incisor dental pulp

Developmental Expression Pattern | Published: 29 March 2023

Badam Enkhmandakh1, Dashzeveg Bayarsaihan*,1,2

1Center for Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT, USA, 2Institute for System Genomics, University of Connecticut, Storrs, CT, USA


SOX transcription factors play key roles in cell differentiation and cell fate determination during development. Using single-cell RNA-sequencing data, we examined the expression profiles of Sox genes in the mouse incisor dental pulp. Our analysis showed that Sox4, Sox5, Sox9, Sox11, and Sox12 are mainly expressed in mesenchymal stem/stromal cells (MSCs) representing osteogenic cells at different stages of differentiation. We found that in several MSCs, Sox genes co-expressed with regulatory genes such as Sp7, Satb2, Msx1, Snai2, Dlx1, Twist2, and Tfap2a. In addition, Sox family genes colocalized with Runx2 and Lef1, which are highly enriched in MSCs undergoing osteoblast differentiation. A protein interaction network analysis uncovered that CREBBP, CEBPB, TLE1, TWIST1, and members of the HDAC and SMAD families are interacting partners of RUNX2 and LEF1 during skeletal development. Collectively, the distinct expression patterns of the SOX transcription factors suggest that they play essential regulatory roles in directing lineage-specific gene expression during differentiation of MSCs.


dental pulp, single cells, chromatin, incisors, transcriptome

This article requires institutional access

Your institutional access has not been identified. Please log in