Enucleolation and nucleolus transfer in mammalian oocytes and zygotes
Published: 16 April 2019
Michal Benc*,1,2, Josef Fulka, Jr.1, František Strejček3, Martin Morovič3, Matej Murín4, Stanislava Martínková1, Dominika Jettmarová1 and Jozef Laurinčík2,4
1Institute of Animal Science, Prague, Czech Republic, 2Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic, 3Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovak Republic and 4The Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Liběchov, Czech Republic
The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.