TY  - JOUR
TI  - Enucleolation and nucleolus transfer in mammalian oocytes and zygotes
AU  - Benc, Michal
AU  - Fulka, JosefJr.
AU  - Strejček, František
AU  - Morovič, Martin
AU  - Murín, Matej
AU  - Martínková, Stanislava
AU  - Jettmarová, Dominika
AU  - Laurinčík, Jozef
T2  - The International Journal of Developmental Biology
AB  - The oocyte GV/GVs (germinal vesicle/germinal vesicles) and zygot PN/PNs (pronucleus/pronuclei) of some mammals contain clearly visible nucleoli which exhibit an atypical morphological structure. These nucleoli (NCLs) can be relatively easily manipulated, i.e. removed from GVs/PNs or eventually transferred into another oocyte/zygote. Thus, with the help of micromanipulation techniques it was possible to uncover the real function(s) they play in processes of oocyte maturation and early embryonic development. The purpose of our review is to describe briefly the micromanipulation techniques that can be used for oocyte/zygote nucleoli manipulation. Moreover, we present some examples of results that were obtained in nucleolus manipulation experiments.
PY  - 2019
DO  - 10.1387/ijdb.190002mb
VL  - 63
IS  - 3-4-5
SP  - 253
EP  - 258
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/190002mb
Y2  - 2024/11/05/15:24:25
ER  -