Int. J. Dev. Biol. 60: 337 - 419 (2016)

https://doi.org/10.1387/ijdb.160274id

Vol 60, Issue 10-11-12

Special Issue: Atlas of Human Experimental Teratomas

Teratomas produced from human pluripotent stem cells xenografted into immunodeficient mice - a histopathology atlas

Open Access | Published: 9 December 2016

Ivan Damjanov*^,1 and Peter W. Andrews²

¹Department of Pathology, The University of Kansas School of Medicine, Kansas City, Kansas, USA and ²The Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Sheffield, UK.

Abstract

This atlas illustrates the microscopic features of tumors produced from human pluripotent stem cells (hPSCs) xenografted into immunosuppressed mice, according to the generally accepted protocols for performing this teratoma assay of stem cell pluripotency. Microphotographs depict various hematoxylin and eosin (H&E) stained tissues derived from all three embryonic germ layers (ectoderm, mesoderm and endoderm). The appearance of persistent hPSC in teratomas is also described with special emphasis on the morphogenesis of embryoid bodies and yolk sac components surrounding them. The use of immunohistochemistry for analyzing hPSC-derived teratomas is also illustrated.

Keywords

human pluripotent stem cells, ectoderm, mesoderm, endoderm, yolk sac