Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production
Published: 19 May 2016
Amin S.M. Salehi, Conner C. Earl, Christina Muhlestein and Bradley C. Bundy*
Department of Chemical Engineering, Brigham Young University, Provo, UT, USA
Cell-free protein synthesis has been around for decades but it has never been close to becoming a robust tool for the production of biotherapeutic agents. In this review, we focus on how Escherichia coli-based cell-free protein synthesis can be modified in various ways to produce challenging, complex anticancer biotherapeutics. Here we report progress in extract preparation and its relation to cell-free cancer research. The future prospects of cell-free technology and its potential in various areas of cancer therapeutics production are also highlighted.
cell-free protein synthesis, cell extract, cancer research, anticancer biotherapeutics