The International Journal of Developmental Biology

Int. J. Dev. Biol. 60: 237 - 243 (2016)

Vol 60, Issue 7-8-9

Special Issue: Cell-free Extracts in Development & Cancer Research

Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production

Published: 19 May 2016

Amin S.M. Salehi, Conner C. Earl, Christina Muhlestein and Bradley C. Bundy*

Department of Chemical Engineering, Brigham Young University, Provo, UT, USA


Cell-free protein synthesis has been around for decades but it has never been close to becoming a robust tool for the production of biotherapeutic agents. In this review, we focus on how Escherichia coli-based cell-free protein synthesis can be modified in various ways to produce challenging, complex anticancer biotherapeutics. Here we report progress in extract preparation and its relation to cell-free cancer research. The future prospects of cell-free technology and its potential in various areas of cancer therapeutics production are also highlighted.


cell-free protein synthesis, cell extract, cancer research, anticancer biotherapeutics

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