The International Journal of Developmental Biology

Volume 60 > Issue 7-8-9 (Special Issue)

Cover Vol. 60 N. 7-8-9

Cell-free Extracts in Development & Cancer Research

Edited by: Jacek Z. Kubiak

Cover Legend

Cell free-extract production from Xenopus laevis eggs or embryos in a nutshell. Inserts on the left, from the top to the bottom: a Xenopus laevis female with eggs laid overnight; harvested eggs in a beaker; and three layers obtained after the first centrifugation (lipids on the top, cytoplasmic extract in the intermediate fraction and the pigment, DNA and cellular debris at the bottom). The main image shows the final step of cell-free extract purification: the clear extract is separated from leftover pigment after the final centrifugation. Photos by Mikolaj Cup (MISMaP, University of Warsaw, Poland and IGDR, University Rennes 1, France).


Cell-free extracts in Development and Cancer Research for over 40 years

Jacek Z. Kubiak

Int. J. Dev. Biol. (2016) 60: 189-191


MPF, starfish oocyte and cell-free extract in the background - an interview with Takeo Kishimoto

Jacek Z. Kubiak and Takeo Kishimoto

Int. J. Dev. Biol. (2016) 60: 193-200

Introductory Papers

Xenopus cell-free extracts and their contribution to the study of DNA replication and other complex biological processes

J. Julian Blow and Ronald A. Laskey

Int. J. Dev. Biol. (2016) 60: 201-207

A sea urchin cell-free system to study male pronuclear assembly and activation

Dominic Poccia

Int. J. Dev. Biol. (2016) 60: 209-219

Studying essential DNA metabolism proteins in Xenopus egg extract

Vincenzo Sannino, Arun M. Kolinjivadi, Giorgio Baldi and Vincenzo Costanzo

Int. J. Dev. Biol. (2016) 60: 221-227

Review Articles

Cell-free Xenopus egg extracts for studying DNA damage response pathways

Steven Cupello, Christine Richardson and Shan Yan

Int. J. Dev. Biol. (2016) 60: 229-236

Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production

Amin S.M. Salehi, Conner C. Earl, Christina Muhlestein and Bradley C. Bundy

Int. J. Dev. Biol. (2016) 60: 237-243

The master Greatwall kinase, a critical regulator of mitosis and meiosis

Suzanne Vigneron, Perle Robert, Khaled Hached, Lena Sundermann, Sophie Charrasse, Jean-Claude Labbé, Anna Castro and Thierry Lorca

Int. J. Dev. Biol. (2016) 60: 245-254

Aurora-A: an expedition to the pole of the spindle in Xenopus egg extracts

Jacek Z. Kubiak and Claude Prigent

Int. J. Dev. Biol. (2016) 60: 255-261

Ubiquitin-mediated proteolysis in Xenopus extract

Gary S. McDowell and Anna Philpott

Int. J. Dev. Biol. (2016) 60: 263-270

Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis

Takashi Onikubo and David Shechter

Int. J. Dev. Biol. (2016) 60: 271-276

Use of Xenopus cell-free extracts to study size regulation of subcellular structures

Predrag Jevtić, Ana Milunović-Jevtić, Matthew R. Dilsaver, Jesse C. Gatlin and Daniel L. Levy

Int. J. Dev. Biol. (2016) 60: 277-288

Reprogramming of somatic cells and nuclei by Xenopus oocyte and egg extracts

Alexander A. Tokmakov, Tetsushi Iwasaki, Ken-Ichi Sato and Shinji Kamada

Int. J. Dev. Biol. (2016) 60: 289-296

Original Articles


Single-molecule, antibody-free fluorescent visualisation of replication tracts along barcoded DNA molecules

Francesco De Carli, Vincent Gaggioli, Gaël A. Millot and Olivier Hyrien

Int. J. Dev. Biol. (2016) 60: 297-304

Flexibility vs. robustness in cell cycle regulation of timing of M-phase entry in Xenopus laevis embryo cell-free extract

Mateusz Debowski, Mohammed El Dika, Jacek Malejczyk, Robert Zdanowski, Claude Prigent, Jean-Pierre Tassan, Malgorzata Kloc, Miroslaw Lachowicz and Jacek Z. Kubiak

Int. J. Dev. Biol. (2016) 60: 305-314

Chromatin assembly and transcriptional cross-talk in Xenopus laevis oocyte and egg extracts

Wei-Lin Wang and David Shechter

Int. J. Dev. Biol. (2016) 60: 315-320