Edited by: Jacek Z. Kubiak
Cell free-extract production from Xenopus laevis eggs or embryos in a nutshell. Inserts on the left, from the top to the bottom: a Xenopus laevis female with eggs laid overnight; harvested eggs in a beaker; and three layers obtained after the first centrifugation (lipids on the top, cytoplasmic extract in the intermediate fraction and the pigment, DNA and cellular debris at the bottom). The main image shows the final step of cell-free extract purification: the clear extract is separated from leftover pigment after the final centrifugation. Photos by Mikolaj Cup (MISMaP, University of Warsaw, Poland and IGDR, University Rennes 1, France).
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