Int. J. Dev. Biol. 59: 391 - 398 (2015)

https://doi.org/10.1387/ijdb.150213gb

Vol 59, Issue 7-8-9

Special Issue: Ionic Messengers in Development and Cancer

HA14-1 potentiates apoptosis in B-cell cancer cells sensitive to a peptide disrupting IP₃ receptor / Bcl-2 complexes

Published: 19 November 2015

Haidar Akl¹, Rita M.L. La Rovere^1,2, Ann Janssens³, Peter Vandenberghe⁴, Jan B. Parys*^,1 and Geert Bultynck*^,1

¹KU Leuven, Lab. Molecular and Cellular Signaling, Dept. Cellular and Molecular Medicine, Leuven, Belgium, ²University “G. d’Annunzio”, Dept. Neuroscience Imaging and Clinical Sciences, Chieti, Italy and ³UZ Leuven, Dept. Hematology, Leuven, Belgium and ⁴KU Leuven, Dept. Human Genetics, Leuven, Belgium

Abstract

Anti-apoptotic B-cell lymphoma 2 (Bcl-2) is commonly upregulated in hematological cancers, including B-cell chronic lymphocytic leukemia (B-CLL) and diffuse large B-cell lymphoma (DLBCL), thereby protecting neoplastic cells from oncogenic-stress-induced apoptosis. Bcl-2 executes its anti-apoptotic function at two different sites in the cell. At the mitochondria, Bcl-2 via its hydrophobic cleft interacts with pro-apoptotic Bcl-2 family members to inhibit apoptosis. At the endoplasmic reticulum (ER), Bcl-2 via its Bcl-2 homology (BH)4 domain, prevents excessive Ca²⁺ signals by interacting with the inositol 1,4,5-trisphosphate receptor (IP₃R), an intracellular Ca²⁺-release channel. A peptide tool (BIRD-2) that targets the BH4 domain of Bcl-2 reverses Bcl-2’s inhibitory action on IP₃Rs and can trigger pro-apoptotic Ca²⁺ signals in B-cell cancer cells. Here, we explored whether HA14-1, a Bcl-2 inhibitor that also inhibits sarco/endoplasmic reticulum Ca²⁺-ATPases (SERCA), could potentiate BIRD-2-induced cell death. We measured apoptosis in Annexin V/7-AAD stained cells using flow cytometry and intracellular Ca²⁺ signals in Fura2-AM-loaded cells using an automated fluorescent plate reader. HA14-1 potentiated BIRD-2-induced Ca²⁺ release from the ER and apoptosis in both BIRD-2-sensitive DLBCL cell lines (SU-DHL-4) and in primary B-CLL cells. BIRD-2-resistant DLBCL cells (OCI-LY-1) were already very sensitive to HA14-1. Yet, although BIRD-2 moderately increased Ca²⁺ levels in HA14-1-treated cells, apoptosis was not potentiated by BIRD-2 in these cells. These results further underpin the relevance of IP₃R-mediated Ca²⁺ signaling as a therapeutic target in the treatment of Bcl-2-dependent B-cell malignancies and the advantage of combination regimens with HA14-1 to enhance BIRD-2-induced cell death.

Keywords

cancer, apoptosis, Bcl-2, IP₃R, Ca²⁺