TY  - JOUR
TI  - HA14-1 potentiates apoptosis in B-cell cancer cells sensitive to a peptide disrupting IP<SUB>3</SUB> receptor / Bcl-2 complexes
AU  - Akl, Haidar
AU  - La Rovere, RitaM.L.
AU  - Janssens, Ann
AU  - Vandenberghe, Peter
AU  - Parys, JanB.
AU  - Bultynck, Geert
T2  - The International Journal of Developmental Biology
AB  - Anti-apoptotic B-cell lymphoma 2 (Bcl-2) is commonly upregulated in hematological cancers, including B-cell chronic lymphocytic leukemia (B-CLL) and diffuse large B-cell lymphoma (DLBCL), thereby protecting neoplastic cells from oncogenic-stress-induced apoptosis. Bcl-2 executes its anti-apoptotic function at two different sites in the cell. At the mitochondria, Bcl-2 via its hydrophobic cleft interacts with pro-apoptotic Bcl-2 family members to inhibit apoptosis. At the endoplasmic reticulum (ER), Bcl-2 via its Bcl-2 homology (BH)4 domain, prevents excessive Ca2+ signals by interacting with the inositol 1,4,5-trisphosphate receptor (IP3R), an intracellular Ca2+-release channel.  A peptide tool (BIRD-2) that targets the BH4 domain of Bcl-2 reverses Bcl-2’s inhibitory action on IP3Rs and can trigger pro-apoptotic Ca2+ signals in B-cell cancer cells. Here, we explored whether HA14-1, a Bcl-2 inhibitor that also inhibits sarco/endoplasmic reticulum Ca2+-ATPases (SERCA), could potentiate BIRD-2-induced cell death. We measured apoptosis in Annexin V/7-AAD stained cells using flow cytometry and intracellular Ca2+ signals in Fura2-AM-loaded cells using an automated fluorescent plate reader. HA14-1 potentiated BIRD-2-induced Ca2+ release from the ER and apoptosis in both BIRD-2-sensitive DLBCL cell lines (SU-DHL-4) and in primary B-CLL cells. BIRD-2-resistant DLBCL cells (OCI-LY-1) were already very sensitive to HA14-1. Yet, although BIRD-2 moderately increased Ca2+ levels in HA14-1-treated cells, apoptosis was not potentiated by BIRD-2 in these cells.  These results further underpin the relevance of IP3R-mediated Ca2+ signaling as a therapeutic target in the treatment of Bcl-2-dependent B-cell malignancies and the advantage of combination regimens with HA14-1 to enhance BIRD-2-induced cell death.
PY  - 2015
DO  - 10.1387/ijdb.150213gb
VL  - 59
IS  - 7-8-9
SP  - 391
EP  - 398
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/150213gb
Y2  - 2024/11/05/09:16:04
ER  -