The International Journal of Developmental Biology

Int. J. Dev. Biol. 59: 217 - 219 (2015)

Vol 59, Issue 4-5-6

A simple method of image analysis to estimate CAM vascularization by APERIO ImageScope software

Technical Article | Published: 15 October 2015

Christian Marinaccio1 and Domenico Ribatti*,1,2

1Department of Basic Medical Sciences, Neurosciences, and Sensory Organs, University of Bari Medical School and 2 National Cancer Institute “Giovanni Paolo II”, Bari, Italy


The chick chorioallantoic membrane (CAM) assay is a well-established method to test the angiogenic stimulation or inhibition induced by molecules and cells administered onto the CAM. The quantification of blood vessels in the CAM assay relies on a semi-manual image analysis approach which can be time consuming when considering large experimental groups. Therefore we present here a simple and fast volumetric method to inspect differences in vascularization between experimental conditions related to the stimulation and inhibition of CAM angiogenesis based on the Positive Pixel Count algorithm embedded in the APERIO ImageScope software.


chick chorioallantoic membrane assay, image analysis, angiogenesis

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