Int. J. Dev. Biol. 53: 1045 - 1051 (2009)

https://doi.org/10.1387/ijdb.092859lv

Vol 53, Issue 7

Essential validation of gene trap mouse ES cell lines: a test case with the gene Ttrap

Technical Article | Published: 18 June 2009

Liesbeth Vermeire^1,3, Abdelilah Ibrahimi^1,3, Thierry Voet^2,3, Lieve Umans^1,3, Kathleen Coddens^1,3, Annick Francis^1,3, Tom Van de Putte^1,3, Leo A. van Grunsven^1,3, An Zwijsen^1,3, Danny Huylebroeck*^,1,3 and Luc Nelles^1,3

¹Laboratory of Molecular Biology (Celgen) and ²Human Genome Laboratory, Centre of Human Genetics, KULeuven and ³Department of Molecular and Developmental Genetics (VIB11), VIB, Leuven, Belgium

Abstract

Gene trapping in mouse embryonic stem (ES) cells enables near-saturation vector-based insertional mutagenesis across the genome of this model organism. About 135,000 trapped ES cell lines are made available to the scientific community by the International Gene Trap Consortium (IGTC; www.genetrap.org). A search of one of its databases identified an ES cell line (RRS512) with a betaGeo-based gene trap (gt) vector insertion in intron 5 of Ttrap, a gene that encodes an intracellular signalling protein, which is implicated in gastrulation movement and left-right asymmetry in zebrafish embryos. We have determined the exact gt insertion point in the mutant ES cell clone RRS512 and confirmed the production of a chimaeric transcript consisting of the upstream Ttrap exons and the gene trap vector encoded marker/selection fusion sequences. This ES cell line was used to generate heterozygous Ttrap mutant mice, which were further crossed to obtain Ttrap^gt/gt mice. In contrast to Ttrap’s documented essential role during nodal and Smad3 controlled zebrafish early embryogenesis, Ttrap^gt/gt mice were born with a normal Mendelian distribution. However, subsequent analysis of these Ttrap^gt/gt mice has revealed a duplication of the wild-type Ttrap allele that was already present in the RRS512 cell line. Based on our detailed analysis presented here, we suggest an extensive procedure for the characterization of gene trap ES cell lines prior to generating gene trap mice with these.

Keywords

duplication, EapII, ES cell, gene trap, Ttrap