Genetic and molecular analysis of INCURVATA2, a negative regulator of floral homeotic genes in the leaves of Arabidopsis thaliana
Published: 1 June 2001
MR Ponce, JM Barrero, H Candela, P Robles, JM Perez-Perez, P Piqueras, A Martinez-Laborda, JL Micol
Univ Miguel Hernandez, Div Genet, Alicante 03202, Spain; Univ Miguel Hernandez, Inst Bioingn, Alicante 03202, Spain
One of the largest available collections of plant morphological mutants is the Arabidopsis Information Service (AIS) Form Mutants collection. We studied 152 AIS lines already known to display abnormally shaped leaves, finding 22 that exhibited involute, upwardly curled, vegetative leaves, a phenotype that we named Incurvata (Icu). Here we present advances in a positional attempt to clone the INCURVATA2 gene, whose recessive allele icu2 is carried by the AIS line N329, and which was mapped near the lower telomere of chromosome 5. The icu2 mutation causes early flowering and Apetala flowers, together with involute leaves, a phenotypic trait associated to ectopic derepression in the leaves of the AGAMOUS and APETALA3 floral organ identity genes.