Int. J. Dev. Biol. 58: 669 - 676 (2014)

https://doi.org/10.1387/ijdb.140174ss

Vol 58, Issue 9

Cloning, expression and characterization of the ornithine decarboxylase gene from Dictyostelium discoideum

Developmental Expression Pattern | Published: 12 March 2015

Rishikesh Kumar, Sheikh Rafia and Shweta Saran*

School of Life Sciences, Jawaharlal Nehru University, New Delhi, India. ssaran@mail.jnu.ac.in

Abstract

Ornithine decarboxylase (ODC) is a rate limiting enzyme in polyamine synthesis that decarboxylates ornithine to form the diamine putrescine. We report here the isolation, expression and characterization of a homolog of ODC from Dictyostelium discoideum. DdODC is conserved and shows sequence and structural homology with that from human. Both ODC transcript and protein are expressed at all stages of development and show high expression in prestalk/stalk cells. It is cytosolic and predominantly perinuclear in localization. Both overexpression of DdODC and putrescine treatment resulted in inhibition of cell proliferation.

Keywords

D. discoideum, ODC expression, putrescine