TY  - JOUR
TI  - Cloning, expression and characterization of the ornithine decarboxylase gene from <I>Dictyostelium discoideum</I>
AU  - Kumar, Rishikesh
AU  - Rafia, Sheikh
AU  - Saran, Shweta
T2  - The International Journal of Developmental Biology
AB  - Ornithine decarboxylase (ODC) is a rate limiting enzyme in polyamine synthesis that decarboxylates ornithine to form the diamine putrescine. We report here the isolation, expression and characterization of a homolog of ODC from Dictyostelium discoideum. DdODC is conserved and shows sequence and structural homology with that from human. Both ODC transcript and protein are expressed at all stages of development and show high expression in prestalk/stalk cells. It is cytosolic and predominantly perinuclear in localization. Both overexpression of DdODC and putrescine treatment resulted in inhibition of cell proliferation.
PY  - 2014
DO  - 10.1387/ijdb.140174ss
VL  - 58
IS  - 9
SP  - 669
EP  - 676
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/140174ss
Y2  - 2024/11/05/11:24:56
ER  -