Int. J. Dev. Biol. 45: S71 - S72 (2001)

Vol 45, Issue S1

OI-KIP, a cyclin dependent kinase inhibitor, is expressed in developing and adult brain of the Medaka (Oryzias latipes)

Published: 1 June 2001

EMC Suarez, V Nguyen, JS Joly, F Bourrat

Univ Santiago de Compostela, Dept Biol Fundamental, Fac Biol, Santiago De Compostela, Spain; CNRS, Inst Fessard, Jeune Equipe INRA Morphogenese Syst Nerveux Chord, F-75700 Paris, France

Abstract

We have isolated OI-KIP, anew member of CIP/ KIP family of cyclin-dependent kinase inhibitors (CDIs), from an expression screen in the medaka (Teleost), and then analyzed its expression pattern in developing and adult brain by in situ hybridization (ISH). To better define the expression profile of this CDI during cell differentiation, we performed a double labeling with OI-KIP mRNA and antibodies against proliferating cell nuclear antigen (PCNA). In the optic tectum, expression of RNA encoding OI-KIP during early embryogenesis is largely associated with the start of cell differentiation in proliferative zones; later in development, its expression is closely related to cell cycle exit. In the adult brain, transcripts appeared also in postmitotic cells long after they have exited the cell cycle. So, they may play a role in maintenance of the quiescent state throughout life.