Alcian blue-stained whole-mount preparations of Xenopus hind limb showing cartilage patterns of st. 55/56 tadpoles. Top: Control. Bottom: Limb developed after transplantation of a fragment from the distal region of st. 52/53 limb bud into a st. 50 limb bud. In most cases, the transplanted fragment did not severely disturb the cartilage pattern along the proximo-distal axis of the host limb, except for occasional production of supernumerary structures in the distal region as shown by this photograph. The transplanted distal cells tended to participate in tissue formation only at the digit level in the experimental limb. It appeared that the grafted cells moved distally to the region corresponding to their original positional ident ity in the developing host limb bud. For details see "Behavior of cells in artificially made cell aggregates and tissue fragments after grafting to developing hind limb buds in Xenopus laevis" by N. Koibuchi and S. Tochinai in this issue.
Regulation of neural crest cell populations: occurrence, distribution and underlying mechanisms.
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