Int. J. Dev. Biol. 34: 453 - 456 (1990)

Vol 34, Issue 4

The Xenopus laevis Hox 2.1 homeodomain protein is expressed in a narrow band of the hindbrain

Published: 1 December 1990

B G Jegalian and E M De Robertis

Department of Biological Chemistry, University of California School of Medicine, Los Angeles 90024-1737.

Abstract

The expression pattern of the Xenopus homeodomain protein Hox 2.1 during development was determined using an affinity-purified antibody directed against a carboxyterminal peptide. Nuclear staining was detected in a very narrow band of the hindbrain. This pattern was compared to that of the previously described Xenopus gene XIHbox 1 in serial sections and found to be more anterior than the XIHbox 1 long protein expression but overlapping with that of the short protein. Xenopus Hox 2.1 protein expression is restricted to a much narrower antero-posterior band than that reported for mouse Hox 2.1 RNA expression by in situ hybridization.