The International Journal of Developmental Biology

Int. J. Dev. Biol. 35: 203 - 207 (1991)

Vol 35, Issue 3

Special Issue: Developmental Biology in Yugoslavia

Neural growth factor inhibits the growth of rat egg-cylinders cultured in vitro

Published: 1 September 1991

J Stipic, F Skreb, Z Bradamante and N Skreb

Clinic of Neurology, University Hospital, Zagreb, Republic of Croatia, Yugoslavia.


Rat egg-cylinders at the primitive streak stage were grown in modified organ culture for 2 weeks using a chemically defined medium. Differentiation of the epidermis and cartilage was comparable to that in fully serum-supplemented medium, whereas neuroblasts were very scarce. In explants treated either with bovine serum albumin or transferrin, neuroblasts were observed, whereas the addition of NGF did not improve neuroblast differentiation. On the contrary, NGF impaired growth and tissue differentiation when compared with explants grown in serum-free medium.

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