Int. J. Dev. Biol. 36: 73 - 84 (1992)

Vol 36, Issue 1

Special Issue: Developmental Biology in Belgium

Plant protoplasts as genetic tool: selectable markers for developmental studies

Published: 1 March 1992

I Negrutiu, S Hinnisdaels, D Cammaerts, W Cherdshewasart, G Gharti-Chhetri and M Jacobs

Laboratory of Plant Genetics, Vrije Universiteit Brussel, Belgium.

Abstract

Protoplasts have usually been presented as a methodological tool. Even as such, they make possible an impressive array of applications in plant biology. Here we report on the use of protoplast-derived selectable markers in the study of several disturbed genetic systems with obvious effects on plant development: (1) auxotrophic mutants and the control of amino acid biosynthesis and transport in vegetative and reproductive tissues; (2) introgression of alien genetic information across phylogenetic boundaries by protoplast fusion, a consequence of controlled dedifferentiation-redifferentiation processes and attenuated incompatibility reactions in cultured cells; (3) expression (in)stability of foreign genes in transgenic plants during successive meiotic generations and in crosses between independent transformants.