The International Journal of Developmental Biology

Int. J. Dev. Biol. 36: 407 - 412 (1992)

Vol 36, Issue 3

Expression and distribution of regeneration-responsive molecule during normal development of the newt, Cynops pyrrhogaster

Published: 30 November -0001

Y Imokawa and G Eguchi

Department of Developmental Biology, National Institute for Basic Biology, Aichi, Japan.


We have investigated the expression and distribution of the regeneration-responsive molecule, 2NI-36, the loss of which is responsible for initiation of dedifferentiation of dorsal marginal iris pigmented epithelial cells to regenerate a lens. In the process of the normal development of the newt, the expression of 2NI-36 could not be detected in embryos at the early developmental stages, i.e., cleavages, gastrulation and neurulation, nor through later developmental stages to tail-bud, even though organogenesis was occurring. 2NI-36 was not detectable in any tissues until embryos reached developmental stage 40 (before hatching). In hatched larvae around developmental stage 46, strong expression of 2NI-36 was observed in several tissues including the vascular endothelium, the pigmented epithelium and the inner layer of skin epidermis. Moreover, 2NI-36 was present on the cell surface of these tissue cells. In conclusion, when the embryos hatch out to become swimming larvae that can feed by themselves, 2NI-36 begins to be expressed in some kinds of differentiated tissues. These results suggest that the function of 2NI-36 might be related to the completion of morphogenesis in development and also to the stabilization of the differentiated state of newly formed tissue cells.

Full text in web format is not available for this article. Please download the PDF version.