The International Journal of Developmental Biology

Int. J. Dev. Biol. 45: S147 - S148 (2001)

Vol 45, Issue S1

Expression and role of adenylyl cyclases during late development in Dictyostelium discoideum

Published: 30 November -0001

E Alvarez-Curto, ME Meima, P Schaap

Univ Dundee, Dept Biochem, Dundee DD1 5EH, Scotland


Development in the social amoeba Dictyostelium discoideum is characterized by the formation of a multicellular structure with a well defined temporal and spatial pattern. Via the activation of cAMP receptors and protein kinase A, cAMP signalling pathways are responsible for many developmentally regulated gene expression events in the organism. cAMP acts as chemoattractant, as an extracellular morphogen and as intracellular messenger. Three adenylyl cyclases have been described so far, ACA, ACG and ACB (Pitt et al., 1992; Meima and Schaap, 1999; Soderbom et al, 1999). Null mutants of these enzymes show defects in aggregation, spore germination (Van Es et al, 1996) and terminal differentiation respectively. To elucidate their role in pattern formation in more detail we use LacZ-promotor constructs and in situ hybridization to compare the wild type pattern with some of the adenylyl cyclase mutants.

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