TY  - JOUR
TI  - Expression of actin mRNA in embryos of the leech Helobdella triserialis.
AU  - Wedeen, C J
AU  - Figueroa, N B
T2  - The International Journal of Developmental Biology
AB  - A cDNA clone (Htr-actin) containing a 1.48 kb insert corresponding to actin was isolated from a stage 10-11 Helobdella triserialis cDNA library by cross hybidization to a Drosophila melanogaster actin clone. The cDNA is equivalent in size to the adult actin mRNA detected by Northern analysis. Genomic Southern blot analysis revealed that Htr-actin is a single copy gene and is one member of a family of actins in H. triserialis. Protein sequence comparisons revealed that Htr-actin was most similar to actin-1 of the earthworm and like other invertebrate actins, Htr-actin was found to be more similar to mammalian cytoplasmic actins than to mammalian muscle-specific actins. In situ hybridization revealed that at early stages the actin mRNA was distributed primarily in the yolk-deficient cytoplasm of all cells. At later stages, elevated levels were detected over the germinal bands and plate. Following muscle formation, a subset of the actin mRNA expression pattern resembled the immunostaining pattern obtained with the muscle specific antibody, Lan3-14. The Htr-actin transcript was undetectable in the segmental nervous system. These studies lay the groundwork for any future ectopic gene expression studies using the Htr-actin promoter in the leech embryo.
PY  - 1998
VL  - 42
IS  - 4
SP  - 581
EP  - 590
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/9694629
Y2  - 2024/11/05/15:24:00
ER  -