TY  - JOUR
TI  - Differential expression of nucleoside diphosphate kinases (NDPK/NM23) during Xenopus early development.
AU  - Ouatas, T
AU  - Selo, M
AU  - Sadji, Z
AU  - Hourdry, J
AU  - Denis, H
AU  - Mazabraud, A
T2  - The International Journal of Developmental Biology
AB  - In Xenopus laevis, three nucleoside diphosphate kinase (NDPK) monomers have been described (NDPK X1, X2 and X3) (Ouatas et al., 1997). In eucaryotes, this kinase is known as a hetero- or homohexamer. Here, we examine the distribution of the enzyme and its different subunit mRNAs during oogenesis and early embryogenesis of Xenopus laevis, respectively by immunohistofluorescence and whole-mount in situ hybridization. These analyses show that NDPKs and their mRNAs are differentially distributed throughout the oocyte and early embryos with a high level of transcription in somites and brain. We emphasize two points. First, each mRNA displays a distinct subcellular localization in somites, suggesting a complex regulation of NDPK genes both at the transcriptional and translational level and a possible involvement of NDPK X2 homohexamers in the dorsal muscle differentiation. Second, in oocytes and early embryos, the proteins are mainly localized in the nucleus, suggesting a new mechanism for their nuclear import, since they do not possess any known nuclear import sequences.
PY  - 1998
VL  - 42
IS  - 1
SP  - 43
EP  - 52
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/9496785
Y2  - 2024/11/21/21:44:14
ER  -