TY - JOUR
TI - Expression analysis of thg1l during Xenopus laevis development
AU - Martini, Davide
AU - De Cesari, Chiara
AU - Digregorio, Matteo
AU - Muscò, Alessia
AU - Giudetti, Guido
AU - Giannaccini, Martina
AU - Andreazzoli, Massimiliano
T2 - The International Journal of Developmental Biology
AB - The tRNA-histidine guanylyltransferase 1-like (THG1L), also known as induced in high glucose-1 (IHG-1), encodes for an essential mitochondria-associated protein highly conserved throughout evolution, that catalyses the 3′–5′ addition of a guanine to the 5′-end of tRNA-histidine (tRNAHis). Previous data indicated that THG1L plays a crucial role in the regulation of mitochondrial biogenesis and dynamics, in ATP production, and is critically involved in the modulation of apoptosis, cell-cycle progression and survival, as well as in cellular stress responses and redox homeostasis. Dysregulations of THG1L expression play a central role in various pathologies, including nephropathies, and neurodevelopmental disorders often characterized by developmental delay and cerebellar ataxia. Despite the essential role of THG1L, little is known about its expression during vertebrate development. Herein, we examined the detailed spatio-temporal expression of this gene in the developing Xenopus laevis. Our results show that thg1l is maternally inherited and its temporal expression suggests a role during the earliest stages of embryogenesis. Spatially, thg1l mRNA localizes in the ectoderm and marginal zone mesoderm during early stages of development. Then, at tadpole stages, thg1l transcripts mostly localise in neural crests and their derivatives, somites, developing kidney and central nervous system, therefore largely coinciding with territories displaying intense energy metabolism during organogenesis in Xenopus.
PY - 2024
DO - 10.1387/ijdb.240033ma
VL - 68
IS - 2
SP - 85
EP - 91
J2 - Int. J. Dev. Biol.
LA - en
SN - 0214-6282
SN - 1696-3547
UR - https://ijdb.ehu.eus/article/240033ma
Y2 - 2024/08/14/14:53:22
ER -