TY  - JOUR
TI  - The stem cell transcription factor ZFP296 transforms NIH3T3 cells and promotes anchorage-independent growth of cancer cells
AU  - Mizoue, Yumi
AU  - Ikeda, Tomomi
AU  - Ikegami, Takako
AU  - Riabets, Oleksandra
AU  - Oishi, Yoshie
AU  - Tobita, Morikuni
AU  - Akutsu, Hidenori
AU  - Hattori, Koichi
AU  - Heissig, Beate
AU  - Koide, Hiroshi
T2  - The International Journal of Developmental Biology
AB  - Cancer cells and embryonic stem (ES) cells share several biological properties, suggesting that some genes expressed in ES cells may play an important role in cancer cell growth. In this study, we investigated the possible role of zinc finger protein 296 (ZFP296), a transcription factor expressed in ES cells, in cancer development. First, we found that overexpression of Zfp296 in NIH3T3 mouse fibroblasts induced two phenomena indicative of cell transformation: enhanced proliferation under low-serum conditions and anchorage-independent growth. We also found that Zfp296 expression was upregulated in the tumor area of a mouse model of colon carcinogenesis. In addition, the expression levels of ZFP296 in various human cell lines were generally low in normal cells and relatively high in cancer cells. Finally, using a soft agar assay, we found that overexpression of ZFP296 promoted the anchorage-independent growth of cancer cells, while its knockdown had the opposite effect. Overall, these results suggest a possible role of the ES-specific transcription factor ZFP296 in cancer.
PY  - 2023
DO  - 10.1387/ijdb.230143hk
VL  - 67
IS  - 4
SP  - 147
EP  - 153
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/230143hk
Y2  - 2024/05/09/06:34:17
ER  -