TY  - JOUR
TI  - Light microscopy of mammalian gametes and embryos: methods and applications
AU  - Ajduk, Anna
AU  - Szkulmowski, Maciej
T2  - The International Journal of Developmental Biology
AB  - In recent years, we have witnessed an unprecedented advancement of light microscopy techniques which has allowed us to better understand biological processes occurring during oogenesis and early embryonic development in mammals. In short, two modes of cellular imaging are now available: those involving fluorescent labels and those which are fluorophore-free. Fluorescence microscopy, in its various forms, is used predominantly in research, as it provides detailed information about cellular processes; however, it can involove an increased risk of photodamage. Fluorophore-free techniques provide, on the other hand, a smaller amount of biological data but they are safer for cells and therefore can be potentially used in a clinical setting. Here, we review various fluorescence and fluorophore-free visualisation approaches and discuss their applicability in developmental biology and reproductive medicine.
PY  - 2019
DO  - 10.1387/ijdb.180300aa
VL  - 63
IS  - 3-4-5
SP  - 235
EP  - 244
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/180300aa
Y2  - 2024/11/21/13:46:46
ER  -