TY  - JOUR
TI  - Myths vs. FACS: what do we know about planarian stem cell lineages?
AU  - Molinaro, AlyssaM.
AU  - Pearson, BretJ.
T2  - The International Journal of Developmental Biology
AB  - Historically, planarian neoblasts were thought to be a homogeneous population of pluripotent stem cells; however, recent population and single-cell level analyses have refuted this idea. Evidence for lineage commitment at the neoblast level has been provided via a number of independent studies using a variety of methods. In situ hybridization experiments first demonstrated the co-expression of lineage-specific markers in neoblasts (marked by piwi-1 expression) isolated by FACS. Subsequently, single cell transcriptomic analyses of FACS-isolated neoblasts uncovered broad lineage-primed neoblast classes based on the clustering of transcriptional profiles and expression of known tissue-specific markers. Additionally, single neoblast pluripotency (and fate restriction) has been demonstrated by single cell transplantation experiments into neoblast-void animals. Here we look to recount the current status of the planarian neoblast field and offer discussion on the caveats of neoblast biology and how to address them experimentally.
PY  - 2018
DO  - 10.1387/ijdb.180066bp
VL  - 62
IS  - 6-7-8
SP  - 527
EP  - 535
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/180066bp
Y2  - 2024/11/05/11:25:19
ER  -