TY  - JOUR
TI  - MPF, starfish oocyte and cell-free extract in the background - an interview with Takeo Kishimoto 
AU  - Kubiak, JacekZ.
AU  - Kishimoto, Takeo
T2  - The International Journal of Developmental Biology
AB  - Professor Takeo Kishimoto’s research has an enormous impact on the cell cycle field. Although his favorite model has always been a starfish oocyte, he has used many other model organisms in his research. Cell-free extracts have been wildly used in his laboratory as a very useful tool to answer cell cycle research questions. Recently, professor Kishimoto discovered the identity of the M-phase promoting factor (MPF) that was thought for years to be cyclin-dependent kinase 1 (CDK1). However, Takeo Kishimoto found that MPF consists in fact of two kinases: CDK1 and Greatwall kinase. While CDK1 phosphorylates mitotic substrates, Greatwall kinase allows these substrates to persist in their phosphorylated state because it regulates phosphatase PP2A, which dephosphorylates the majority of CDK1 substrates. When I started to interview Prof. Kishimoto, I was mostly interested in his experiences with cell-free extracts. However, as you will see below we almost immediately turned to the problem of the identity of MPF. This is fully understandable because the identity of MPF seems to be a major interest in Takeo’s scientific career. I hope readers will enjoy this interview and will be able to learn about many aspects of scientific research, which do not usually appear in regular research papers.
PY  - 2016
DO  - 10.1387/ijdb.160348jk
VL  - 60
IS  - 7-8-9
SP  - 193
EP  - 200
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/160348jk
Y2  - 2024/11/23/13:37:06
ER  -