TY  - JOUR
TI  - Identification of a second Xenopus twisted gastrulation gene.
AU  - Oelgeschlager, Michael
AU  - Tran, Uyen
AU  - Grubisic, Kristina
AU  - De Robertis, Edward M
T2  - The International Journal of Developmental Biology
AB  - Twisted Gastrulation (Tsg) is a secreted molecule which regulates BMP signalling in the extracellular space as part of an evolutionarily conserved network of interacting proteins. In Xenopus, maternal xTsg mRNA can be found throughout the early embryo. After gastrulation, xTsg is expressed as part of the BMP4 synexpression group until late tadpole stages. Here we report the identification of a second Xenopus Tsg gene (xTsg-2). Xenopus Tsg-2 is highly homologousto xTsg. In particular, amino acid residues which have been shown to be required for the binding of xTsg to BMP and to Chordin are conserved. The expression of Xenopus Tsg-2 mRNA was restricted to late stages of embryonic development; it was detected at tadpole stages in lateral plate mesoderm, neural crest, branchial arches and head mesenchyme. In microinjection experiments, the activity of xTsg-2 mRNA was similar to that of xTsg. We conclude that two Tsg genes act in distinct temporal and spatial territories in the course of Xenopus embryonic development.
PY  - 2004
DO  - 10.1387/ijdb.15005575
VL  - 48
IS  - 1
SP  - 57
EP  - 61
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/15005575
Y2  - 2024/05/17/08:52:15
ER  -