TY  - JOUR
TI  - Identification of distal enhancers for <I>Six2</I> expression in pronephros
AU  - Suzuki, Nanoka
AU  - Hirano, Kodai
AU  - Ogino, Hajime
AU  - Ochi, Haruki
T2  - The International Journal of Developmental Biology
AB  - The embryonic nephric mesenchyme contains pluripotent progenitor cells. Six2, a homeodomain transcription factor, is expressed in a subset of the nephric mesenchyme, and it functions to maintain a progenitor state by suppressing nephrogenesis. Despite the functional significance of Six2 in nephric development, its regulatory mechanisms remain unclear. To identify the cis-regulatory elements for Six2, we focused on the evolutionarily conserved sequences known as conserved noncoding sequences (CNSs) associated with the Six2 locus. Transgenic experiments using Xenopus laevis embryos revealed that three of the eight CNSs located within a 317-kb segment of the Six2 genomic locus were nephric enhancers. Motif analysis of transcription factors combined with phylogenetic footprinting revealed the enrichment of putative T-cell factor (Tcf)-, Hox-, and SWI/SNF complex helicase-like transcription factor (Hltf)- and AT-rich interactive domain 3A (Arid3a)-binding motif sequences in these enhancers.
PY  - 2015
DO  - 10.1387/ijdb.140263ho
VL  - 59
IS  - 4-5-6
SP  - 241
EP  - 246
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/140263ho
Y2  - 2024/11/21/17:38:37
ER  -