TY - JOUR TI - Transformation of leguminous plants to study symbiotic interactions AU - Iantcheva, Anelia AU - Mysore, KirankumarS. AU - Ratet, Pascal T2 - The International Journal of Developmental Biology AB - Legume plants are important in agriculture because they represent an important source of protein for human and animal consumption. This high protein content results from their capacity to use atmospheric nitrogen for their nutrition as a consequence of their symbiotic interaction with rhizobia. Understanding this interaction at the molecular level is a prerequisite for its better use in agriculture and for the long term objective of its transfer to other crops. Agrobacterium-mediated transformation is a tool of choice for studying this interaction and for unraveling the function of the different genes discovered through classical genetic approaches. However, legume plants are often recalcitrant to regeneration and transformation. This paper describes the technology developments (regeneration, transformation, insertion mutagenesis) related to Agrobacterium transformations that were established in the legume plants, as well as different examples of the technology developments or gene discoveries resulting from these studies. PY - 2013 DO - 10.1387/ijdb.130239pr VL - 57 IS - 6-7-8 SP - 577 EP - 586 J2 - Int. J. Dev. Biol. LA - en SN - 0214-6282 SN - 1696-3547 UR - https://ijdb.ehu.eus/article/130239pr Y2 - 2024/11/05/17:18:33 ER -