TY - JOUR TI - From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes AU - Marton, Ira AU - Honig, Arik AU - Omid, Ayelet AU - De Costa, Noam AU - Marhevka, Elena AU - Cohen, Barry AU - Zuker, Amir AU - Vainstein, Alexander T2 - The International Journal of Developmental Biology AB - Researchers and biotechnologists require methods to accurately modify the genome of higher eukaryotic cells. Such modifications include, but are not limited to, site-specific mutagenesis, site-specific insertion of foreign DNA, and replacement and deletion of native sequences. Accurate genome modifications in plant species have been rather limited, with only a handful of plant species and genes being modified through the use of early genome-editing techniques. The development of rare-cutting restriction enzymes as a tool for the induction of site-specific genomic double-strand breaks and their introduction as a reliable tool for genome modification in animals, animal cells and human cell lines have paved the way for the adaptation of rare-cutting restriction enzymes to genome editing in plant cells. Indeed, the number of plant species and genes which have been successfully edited using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and engineered homing endonucleases is on the rise. In our review, we discuss the basics of rare-cutting restriction enzyme-mediated genome-editing technology with an emphasis on its application in plant species. PY - 2013 DO - 10.1387/ijdb.130205av VL - 57 IS - 6-7-8 SP - 639 EP - 650 J2 - Int. J. Dev. Biol. LA - en SN - 0214-6282 SN - 1696-3547 UR - https://ijdb.ehu.eus/article/130205av Y2 - 2024/11/05/15:24:10 ER -