TY  - JOUR
TI  - Fundamental discoveries and simple recombination between circular plasmid DNAs led to widespread use of <I>Agrobacterium</I> <I>tumefaciens</I> as a generalized vector for plant genetic engineering
AU  - Zambryski, Patricia
T2  - The International Journal of Developmental Biology
AB  - Fundamental research aimed to determine the limits of the Agrobacterium transfer DNA (T-DNA) element that stably inserted into plant nuclear DNA to cause crown gall tumor formation. The T-DNA borders were discovered to be exceedingly precise, revealing that T-DNA insertion into the plant genome was reproducible and exact. Deletion of the internal regions of the T-DNA, to remove the tumor forming genes, while retaining the T-DNA borders, resulted again in efficient DNA transfer to plant cells, but now such cells were capable of completely normal growth and differentiation. Thus, the internal region of the T-DNA was not needed for DNA transfer, and one could envisage insertion of any DNA of interest in between the T-DNA borders. Thus began plant genetic engineering.
PY  - 2013
DO  - 10.1387/ijdb.130190pz
VL  - 57
IS  - 6-7-8
SP  - 449
EP  - 452
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/130190pz
Y2  - 2024/11/21/18:05:04
ER  -