TY  - JOUR
TI  - Identification of cis-elements regulating expression of Fgf10 during limb development.
AU  - Sasak, Hidemi
AU  - Yamaoka, Takashi
AU  - Ohuchi, Hideyo
AU  - Yasue, Akihiro
AU  - Nohno, Tsutomu
AU  - Kawano, Hirotaka
AU  - Kato, Shigeaki
AU  - Itakura, Mitsuo
AU  - Nagayama, Masaru
AU  - Noji, Sumihare
T2  - The International Journal of Developmental Biology
AB  - Fibroblast growth factor 10 (FGF10) is known to be expressed in limb mesenchymal cells and to function as a mesenchymal signaling factor involved in epithelial-mesenchymal interactions during limb development. To elucidate regulation of Fgf10 expression, we isolated the promoter region of Fgf10 containing its 2.0 kb upstream 5'-fragment from the initiation codon and its 0.9 kb downstream fragment. Transcriptional activity of the fragment was examined with transgenic mice, using a lacZ-reporter system. Although no significant expression of the reporter gene was observed for the 0.2 kb 5'-fragment, expression was detected in the apical ectodermal ridge of the limb bud and developing cartilage of the limb for the 2.0 kb and 0.7 kb 5'-fragments, respectively. From comparison of the mouse sequences of the 2.0 kb fragment with corresponding sequences of human and chicken Fgf10, we identified 17 conserved putative enhancer motifs for AER expression and other unidentified expressions. For limb cartilage expression, we found putative enhancer sequences conserved among the three species in the 0.7 kb 5'-fragment. In the fragment, three DNA binding motifs were identified in the mouse and human sequence, although they are not conserved in the corresponding chicken sequence.
PY  - 2002
VL  - 46
IS  - 7
SP  - 963
EP  - 967
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/12455635
Y2  - 2024/11/21/17:59:53
ER  -