TY  - JOUR
TI  - Selecting karyophilic DNA cis elements in Xenopus laevis oocytes; a new approach.
AU  - Marcello, Marco
AU  - Fischer, Roger
AU  - Troster, Helmut
AU  - Trendelenburg, Michael F
AU  - Sczakiel, Georg
T2  - The International Journal of Developmental Biology
AB  - The intracellular localisation and mobility of exogenous DNA introduced into Xenopus laevis oocytes is largely unknown. In this paper, we report a new technique to investigate the cytoplasmic/nuclear transport of a random pool of linear, double-stranded, oligomeric DNA of 147 bp in length. We chose a combinatorial approach which made use of repetitive rounds of selection and amplification to search for new cis elements mediating nuclear import or retention. A new PCR-based methodology was established to reliably detect exogenous DNA in subcellular and total extracts prepared from Xenopus laevis oocytes. Studies in vivo and with cellular extracts indicate the presence of a highly efficient nuclease activity in the nuclear compartment. The described combinatorial approach constitutes a promising tool for the isolation of novel DNA cis elements which may play an important role in the nuclear internalisation and retention of exogenous DNA in Xenopus laevis oocytes.
PY  - 2002
VL  - 46
IS  - 3
SP  - 309
EP  - 316
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/12068952
Y2  - 2024/05/02/07:15:11
ER  -