TY  - JOUR
TI  - Histone hyperacetylation during meiosis interferes with large-scale chromatin remodeling, axial chromatid condensation and sister chromatid separation in the mammalian oocyte
AU  - Yang, Feikun
AU  - Baumann, Claudia
AU  - Viveiros, MariaM.
AU  - De La Fuente, Rabindranath
T2  - The International Journal of Developmental Biology
AB  - Histone acetylation regulates higher-order chromatin structure and function and is critical for the control of gene expression. Histone deacetylase inhibitors (HDACi) are currently un¬der investigation as novel cancer therapeutic drugs. Here, we show that female germ cells are extremely suscep¬tible to chromatin changes induced by HDACi. Our results indicate that exposure to trichostatin A (TSA) at nanomolar levels interferes with major chromatin remodeling events in the mammalian oocyte leading to chromosome instability. High resolution analysis of chromatin structure and live-cell imaging revealed a striking euchromatin decondensation associated with histone H4 hyperacetylation following exposure to 15 nM TSA in >90% of pre-ovulatory oocytes. Dynamic changes in large-scale chromatin structure were detected after 2 h of exposure and result in the formation of misaligned chromosomes in >75% (P
PY  - 2012
DO  - 10.1387/ijdb.120246rd
VL  - 56
IS  - 10-11-12
SP  - 889
EP  - 899
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/120246rd
Y2  - 2024/12/28/21:55:53
ER  -