TY  - JOUR
TI  - A screen for genes expressed in Drosophila imaginal discs.
AU  - Gerlitz, Offer
AU  - Nellen, Denise
AU  - Ottiger, Michael
AU  - Basler, Konrad
T2  - The International Journal of Developmental Biology
AB  - The development of Drosophila imaginal discs serves as a model system to understand how genes determine the shape and size of an organ. The identification of genes involved in this process is an important step towards this goal. Here we describe a P-element based enhancer trap screen for genes expressed in the larval imaginal discs. Our aim was to establish a large collection of enhancer trap lines each showing expression of Gal4 in imaginal discs. To this end, we improved the well established P-element vector pGawB in order to obtain higher in vivo transposition frequencies. In addition we chose an F1-screening approach using UAS-GFP as a reporter gene. This system permits the efficient screening of larval and pupal stages of living animals and the detection of imaginal gene expression patterns through the transparent cuticle. The procedure has been optimized for high-throughput. 2'000 P-element insertions have been established which exhibit expression in imaginal discs.
PY  - 2002
VL  - 46
IS  - 1
SP  - 173
EP  - 176
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/11902680
Y2  - 2024/11/05/19:26:17
ER  -