TY  - JOUR
TI  - The role of non-muscle myosin IIA in aggregation and invasion of human MCF-7 breast cancer cells
AU  - Derycke, Lara
AU  - Stove, Christophe
AU  - Vercoutter-Edouart, Anne-Sophie
AU  - De Wever, Olivier
AU  - Dollé, Laurent
AU  - Colpaert, Nathalie
AU  - Depypere, Herman
AU  - Michalski, Jean-Claude
AU  - Bracke, Marc
T2  - The International Journal of Developmental Biology
AB  - Human MCF-7/6 breast cancer cells differ from their MCF-7/AZ counterparts by their invasiveness in a number of assays in vitro, such as invasion of MCF-7 spheroids into embryonic chick heart fragments or type I collagen gels. Comparative proteomic analysis of these two variants revealed an identical pattern, except for a 230 kDa protein present in the invasive MCF-7/6 variant, but hardly detectable in the non-invasive MCF-7/AZ one. This protein appeared to be the non-muscle myosin IIA heavy chain (NMIIA), also coined MYH9. Experimental inhibition of NMIIA by reducing either its expression (via stable shRNA transduction) or its function (via the specific ATPase inhibitor blebbistatin) underpinned the decisive role of NMIIA in MCF-7 cell invasion. Inhibition of NMIIA indeed blocked the invasion of MCF-7/6 cells in three-dimensional invasion substrata such as embryonic chick heart fragments and type I collagen gels. Invasiveness of MCF-7/6 cells has been related to poor formation and compaction of aggregates, due to a functionally defective E-cadherin/catenin complex. Both genetic and pharmacological inhibition of NMIIA stimulated MCF-7/6 cell aggregation. Together, these data indicate that NMIIA is a decisive protein for MCF-7 cells to invade, indicating that this molecule is a candidate for targeted anti-invasive treatment.
PY  - 2011
DO  - 10.1387/ijdb.113336ld
VL  - 55
IS  - 7-8-9
SP  - 835
EP  - 840
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/113336ld
Y2  - 2024/11/21/18:32:43
ER  -