TY  - JOUR
TI  - Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments
AU  - Du Puy, Leonie
AU  - Beqqali, Abdelaziz
AU  - Van Tol, HelenaT.A.
AU  - Monshouwer-Kloots, Jantine
AU  - Passier, Robert
AU  - Haagsman, HenkP.
AU  - Roelen, BernardA.J.
T2  - The International Journal of Developmental Biology
AB  - SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation.
PY  - 2012
DO  - 10.1387/ijdb.113327lp
VL  - 56
IS  - 4
SP  - 301
EP  - 309
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/113327lp
Y2  - 2024/11/24/03:46:24
ER  -