TY  - JOUR
TI  - Co-localization of neural cell adhesion molecule and fibroblast growth factor receptor 2 in early embryo development 
AU  - Vesterlund, Liselotte
AU  - Tohonen, Virpi
AU  - Hovatta, Outi
AU  - Kere, Juha
T2  - The International Journal of Developmental Biology
AB  - During development there is a multitude of signaling events governing the assembly of the developing organism. Receptors for signaling molecules such as fibroblast growth factor receptor 2 (FGFR2) enable the embryo to communicate with the surrounding environment and activate downstream pathways. The neural cell adhesion molecule (NCAM) was first characterized as a cell adhesion molecule highly expressed in the nervous system, but recent studies have shown that it is also a signaling receptor. Using a novel single oocyte adaptation of the proximity ligation assay, we here show a close association between NCAM and FGFR2 in mouse oocytes and 2-cell embryos. Real-time PCR analyses revealed the presence of messenger RNA encoding key proteins in downstream signaling pathways in oocytes and early mouse embryos. In summary these findings show a co-localization of NCAM and FGFR2 in early vertebrate development with intracellular signaling pathways present to enable a cellular response.
PY  - 2011
DO  - 10.1387/ijdb.103240lv
VL  - 55
IS  - 3
SP  - 313
EP  - 319
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/103240lv
Y2  - 2024/11/24/05:51:30
ER  -