TY  - JOUR
TI  - Glucocorticoid receptor antagonizes EGFR function to regulate eyelid development 
AU  - Sanchis, Ana
AU  - Bayo, Pilar
AU  - Sevilla, LisaM.
AU  - Pérez, Paloma
T2  - The International Journal of Developmental Biology
AB  - The glucocorticoid receptor (GR) plays a crucial role in epidermal morphogenesis during embryonic development, as demonstrated by analyzing genetically modified mouse models of GR gain- and loss-of-function. Eyelid formation constitutes a useful model to study epithelial development, as it requires coordinated regulation of keratinocyte proliferation, apoptosis and migration. We have analyzed this biological process in GR-/- embryos during ontogeny. Our data demonstrate that GR deficiency results in delayed and impaired eyelid closure, as illustrated by increased keratinocyte proliferation and apoptosis along with impaired differentiation in GR-/- eyelid epithelial cells. These defects are due, at least in part, to the lack of antagonism between GR and epidermal growth factor receptor (EGFR) signaling, causing sustained activation of the MAPK/AP-1 pathway and the upregulation of keratin K6 at embryonic stage E18.5. Additionally, we demonstrate that GR regulates epithelial cell migration in vitro by interfering with EGFR-mediated signaling. Overall, GR/EGFR antagonism appears as a major mechanism regulating ocular epithelial development.
PY  - 2010
DO  - 10.1387/ijdb.103071as
VL  - 54
IS  - 10
SP  - 1473
EP  - 1480
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/103071as
Y2  - 2024/11/21/23:30:10
ER  -