TY  - JOUR
TI  - Six1 is indispensable for production of functional progenitor cells during olfactory epithelial development 
AU  - Ikeda, Keiko
AU  - Kageyama, Ryoichiro
AU  - Suzuki, Yuko
AU  - Kawakami, Kiyoshi
T2  - The International Journal of Developmental Biology
AB  - The rodent olfactory epithelium (OE) is a good model system for studying the principles of stem and progenitor cell biology, because of its capacity for continuous neurogenesis throughout life and relatively well-characterized neuronal lineage. The development of mouse OE is divided into two stages, early and established neurogenesis. In established neurogenesis, which starts at embryonic day (E) 12.5, sustentacular cells and olfactory receptor neurons (ORNs) are produced from apical and basal progenitors, respectively. We previously reported that Six1-/- shows a lack of mature ORNs throughout development and disorganization of OE after E12.5. However, the molecular bases for these defects have not been addressed. Here, we show that Six1 is expressed in both apical and basal progenitors. In Six1-/- mice, apical proliferating cells were absent and no morphologically identifiable sustentacular cells were observed. Consistently, the expression of Notch2 and Jagged1 in the apical layer was absent in Six1-/- mice. On the other hand, basal proliferating cells were observed in Six1-/- animals, but the expression of Ngn1, NeuroD, Notch1, and Jagged2 in the basal layer was absent. The expression of Mash1, the determination gene for ORNs, and Hes genes was enhanced in Six1-/- mice. The present findings suggest that Six1 regulates production of functional apical and basal progenitors during OE development, through the regulation of various genes, such as neuronal basic helix-loop-helix (bHLH), neuronal repressor bHLH, and genes involved in the Notch signaling pathway. 
PY  - 2010
DO  - 10.1387/ijdb.093041ki
VL  - 54
IS  - 10
SP  - 1453
EP  - 1464
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/093041ki
Y2  - 2024/11/21/22:26:13
ER  -