TY  - JOUR
TI  - Characterization of an <I>lhx1a</I> transgenic reporter in zebrafish 
AU  - Swanhart, LisaM.
AU  - Takahashi, Nobuhiro
AU  - Jackson, RachelL.
AU  - Gibson, GregoryA.
AU  - Watkins, SimonC.
AU  - Dawid, IgorB.
AU  - Hukriede, NeilA.
T2  - The International Journal of Developmental Biology
AB  - The LIM-domain containing transcription factor, Lhx1, is involved in the regulation of early gastrulation cell movements, kidney organogenesis and other processes in vertebrate model organisms. To follow the expression of this gene in live embryos, we created transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of lhx1a regulatory regions. Tg(lhx1a:EGFP) pt303 recapitulates the expression of endogenous lhx1a beginning at early gastrula stages through 72 hours of development with only few exceptions. In addition, over-expression of the Nodal ligand, ndr1, results in the concomitant expansion of the transgene and endogenous lhx1a expression. Treatment of Tg(lhx1a:EGFP) pt303 embryos with the small molecule SB-431542, an inhibitor of Nodal signaling, results in the loss of both transgene and endogenous lhx1a expression. These experiments suggest that Tg(lhx1a:EGFP) pt303 is regulated in a manner similar to endogenous lhx1a. Therefore, this reporter can be utilized not only for monitoring lhx1a expression, but also for numerous applications, including chemical genetics screening.
PY  - 2010
DO  - 10.1387/ijdb.092969ls
VL  - 54
IS  - 4
SP  - 731
EP  - 736
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/092969ls
Y2  - 2024/11/05/13:18:30
ER  -