TY  - JOUR
TI  - Modeling and quantification of cancer cell invasion through collagen type I matrices 
AU  - De Wever, Olivier
AU  - Hendrix, An
AU  - De Boeck, Astrid
AU  - Westbroek, Wendy
AU  - Braems, Geert
AU  - Emami, Shahin
AU  - Sabbah, Michèle
AU  - Gespach, Christian
AU  - Bracke, Marc
T2  - The International Journal of Developmental Biology
AB  - Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively. 
PY  - 2010
DO  - 10.1387/ijdb.092948ow
VL  - 54
IS  - 5
SP  - 887
EP  - 896
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/092948ow
Y2  - 2024/11/05/13:15:11
ER  -