TY  - JOUR
TI  - <I>XRASGRP2</I> is essential for blood vessel formation during <I>Xenopus</I> development 
AU  - Suzuki, Kan
AU  - Takahashi, Shuji
AU  - Haramoto, Yoshikazu
AU  - Onuma, Yasuko
AU  - Nagamine, Kentaro
AU  - Okabayashi, Koji
AU  - Hashizume, Kohei
AU  - Iwanaka, Tadashi
AU  - Asashima, Makoto
T2  - The International Journal of Developmental Biology
AB  - Ras guanyl nucleotide-releasing protein 2 (RASGRP2), one of the Ras guanine exchange factors, is implicated as a critical regulator of inside-out integrin activation in human lymphocytes, neutrophils and platelets. However, the activities of this protein in endothelial cells remain unclear. In the current study, we identify a physiological function in blood vessel formation for XRASGRP2, which is the Xenopus ortholog of mammalian RASGRP2. XRASGRP2 over-expression induced ectopic vascular formation, and XRASGRP2-knockdown embryos showed delayed vascular development. We also investigated the upstream signaling of XRASGRP2 in endothelium formation. XRASGRP2 expression was up-regulated in the presence of VEGF-A and down-regulated following VEGF-A depletion. XRASGRP2 knockdown abolished the ectopic induction of endothelial cells by VEGF-A in the posterior ventral blood island. These results suggest that XRASGRP2 is essential for vascular formation during Xenopus development.
PY  - 2010
DO  - 10.1387/ijdb.092929ks
VL  - 54
IS  - 4
SP  - 609
EP  - 615
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/092929ks
Y2  - 2024/11/21/18:06:06
ER  -