TY  - JOUR
TI  - Characterization of fibroblast-like cells from the rat olfactory bulb 
AU  - Soleimani, Masoud
AU  - Nadri, Samad
AU  - Salehi, Mohammad
AU  - Sobhani, Aligholi
AU  - Hajarizadeh, Athena
T2  - The International Journal of Developmental Biology
AB  - The isolation and characterization of stem cells from an alternative tissue is a subject of intensive investigation. In the present study, we have focused on the characterization of fibroblastic cells in olfactory bulb tissue of the rat. To this end,  4-6 week old rats were killed and their olfactory bulb tissue was dissected out. Olfactory bulb derived fibroblast-like cells were recovered by adhesion to cell culture plastic. The plastic adherent cultivated cells were induced to differentiate along osteoblastic, adipogenic and chondrogenic lineages. Furthermore, the expression of some surface antigens was investigated. We obtained purified cells with spindle shaped morphology in primary culture, which differentiated into mesenchymal lineages. These cells expressed CD29 and CD90 (Thy1.1) surface antigens, but not CD31, CD34 and CD45. Our results indicate that fibroblast-like cells from the olfactory bulb are mesenchymal stem cells in nature. Taken together, our data suggest that olfactory bulb tissue may constitute a new source of mesenchymal stem cells and could be used for the treatment of injury.
PY  - 2008
DO  - 10.1387/ijdb.082710ms
VL  - 52
IS  - 7
SP  - 979
EP  - 984
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/082710ms
Y2  - 2024/11/24/16:17:30
ER  -