TY  - JOUR
TI  - A histone H1 variant is required for erythrocyte maturation in medaka
AU  - Matsuoka, Osamu
AU  - Shindo, Norihisa
AU  - Arai, Daisuke
AU  - Higashinakagawa, Toru
T2  - The International Journal of Developmental Biology
AB  - Three histone H1 variants were identified in medaka fish and their sequence characteristics were analyzed. This paper reports one of these variants, termed H1-2, because of its possible implication in erythrocyte maturation. The amino acid sequence of H1-2 was phylogenetically similar to that of other replication-dependent histones. The mRNA transcribed from the h1-2  gene, however, possessed a poly(A) tail without a stem-loop structure, indicating that H1-2 may represent a replication-independent (RI) histone. Transcripts from the h1-2 gene were largely localized in erythrocytes, and knock-down of the h1-2 gene with morpholino antisense oligos resulted in failure to develop mature erythrocytes. In the morphants, residual erythrocytes showed severely impaired nuclear compaction. Although not structurally related to chicken RI histone H5, which is required for erythrocyte maturation, H1-2 may constitute its functional counterpart. Our findings may offer evolutionary insights into the function of H1 variants.
PY  - 2008
DO  - 10.1387/ijdb.072568om
VL  - 52
IS  - 7
SP  - 887
EP  - 892
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/072568om
Y2  - 2024/11/24/15:19:50
ER  -